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Student Content Assessments

Instrument 20: Beckman-Coulter P/ACE MDQ High Performance Liquid Chromatography (HPLC) Evaluation

Project: Instrumentation for Instruction in Analytical Separations
Trinity University

Funding Source: NSF - Undergraduate education (DUE)

Purpose: To assess the effectiveness of incorporating capillary electrophoresis (CE) throughout the chemistry curriculum.

Administered To: Students using Beckman-Coulter P/ACE MDQ High Performance Liquid Chromatography (HPLC) instrument in a laboratory course.

Topics Covered:

  • Background Characteristics & Activities (Student): experience with the instrument
  • Content Specific Assessment: Chemistry: instrumentation - High Performance Liquid Chromatography (HPLC)

Format/Length: 5 open-ended questions


         This questionnaire is being given to all students using the Beckman-Coulter HPLC instrument in a laboratory course. Your answers on this form will be used to assess the effectiveness of incorporating this instrument throughout the chemistry curriculum. This is a required procedure as described in our grant proposal to the National Science Foundation. The NSF provided funds for 50% of the cost of the HPLC.

         Your answers will not be used to grade your performance in the laboratory course, but rather to grade the NSF project. Your instructor may require that you complete the form by some means but will not grade individual questions. Please answer each question to the best of your ability. Your answers should be based on your knowledge of the technique, whether that was gained in this course or over multiple courses or experiences. If you do not know the answer to a question, simply state that you don't know. We are not interested in grading you but finding out and recording what you know about this instrument and technique. Use as much space as is necessary (use the back of this form or attach extra sheets) to provide a complete answer or the necessary explanations.

         This study has been approved by the [your university/college name here] Institutional Review Board. If you have any questions regarding the approval, please contact Chair [your university/college name here] IRB (999-xxxx). Your answers will be kept confidential. Your participation involves no risks or benefits to you.

Name:________________ Class:____________ Date and Year:___________
1. Please describe your previous (if any) experience with HPLC (High Perfonnance Liquid Chromatography) and its related techniques.




2. What types of analytes can be separated by HPLC?




3. What properties of a molecule help to determine its elution time relative to other components in the sample?




4. What type of infonnation can be obtained about a sample or analyte with a HPLC experiment?




5. If two peaks elute at the same time, how might you try to resolve them?